In situ tissue pathology from spatially encoded mass spectrometry classifiers visualized in real time through augmented reality.

ABSTRACT: Integration between a hand-held mass spectrometry desorption probe based on picosecond infrared laser technology (PIRL-MS) and an optical surgical tracking system demonstrates in situ tissue pathology from point-sampled mass spectrometry data. Spatially encoded pathology classifications are displayed at the site of laser sampling as color-coded pixels in an augmented reality video feed of the surgical field of view. This is enabled by two-way communication between surgical navigation and mass spectrometry data analysis platforms through a custom-built interface. Performance of the system was evaluated using murine models of human cancers sampled in situ in the presence of body fluids with a technical pixel error of 1.0 ± 0.2 mm, suggesting a 84% or 92% (excluding one outlier)

SUBMITTER: Woolman M 

PROVIDER: S-EPMC8163395 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature