Efficient dilution-to-extinction isolation of novel virus-host model systems for fastidious heterotrophic bacteria.

ABSTRACT: Microbes and their associated viruses are key drivers of biogeochemical processes in marine and soil biomes. While viruses of phototrophic cyanobacteria are well-represented in model systems, challenges of isolating marine microbial heterotrophs and their viruses have hampered experimental approaches to quantify the importance of viruses in nutrient recycling. A resurgence in cultivation efforts has improved the availability of fastidious bacteria for hypothesis testing, but this has not been matched by similar efforts to cultivate their associated bacteriophages. Here, we describe a high-throughput method for isolating important virus-host systems for fastidious heterotrophic bacteria that couples advances in culturing of hosts with sequential enrichment and isolation of associated phages

SUBMITTER: Buchholz HH 

PROVIDER: S-EPMC8163748 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature