Proteotyping of knockout mouse strains reveals sex- and strain-specific signatures in blood plasma.

ABSTRACT: We proteotyped blood plasma from 30 mouse knockout strains and corresponding wild-type mice from the International Mouse Phenotyping Consortium. We used targeted proteomics with internal standards to quantify 375 proteins in 218 samples. Our results provide insights into the manifested effects of each gene knockout at the plasma proteome level. We first investigated possible contamination by erythrocytes during sample preparation and labeled, in one case, up to 11 differential proteins as erythrocyte originated. Second, we showed that differences in baseline protein abundance between female and male mice were evident in all mice, emphasizing the necessity to include both sexes in basic research, target discovery, and preclinical effect and safety studies. Next, we identified the protein si

SUBMITTER: Mohammed Y 

PROVIDER: S-EPMC8163790 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature