Optimisation of sample storage and DNA extraction for human gut microbiota studies​.

ABSTRACT:

Background

New developments in next-generation sequencing technologies and massive data received from this approach open wide prospects for personalised medicine and nutrition studies. Metagenomic analysis of the gut microbiota is paramount for the characterization of human health and wellbeing. Despite the intensive research, there is a huge gap and inconsistency between different studies due to the non-standardised and biased pipeline. Methodical and systemic understanding of every stage in the process is necessary to overcome all bottlenecks and grey zones of gut microbiota studies, where all details and interactions between processes are important.

Results

Here we show that an inexpensive, but reliable iSeq 100 platform is an excellent tool to perform the analysis of the

SUBMITTER: Kazantseva J 

PROVIDER: S-EPMC8164492 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature