Rapid and simultaneous identification of three mutations by the Novaplex™ SARS-CoV-2 variants I assay kit.

ABSTRACT:

Background

. The emergence of SARS-CoV-2 variants has caused an unexpected rebound globally. The World Health Organization has listed three variants (B.1.1.7, B.1.351, and P.1) as variants of concern. To understand the epidemiology and thereby plan appropriate safety measures, differential identification of the variants is indeed critical.

Objectives

. Although whole-genome sequencing is the gold standard for variant identification, it is time-consuming and relatively expensive. Therefore, a rapid, easy, and cost-effective platform targeting multiple regions of the genome is required. Here, we assessed the usefulness of the Novaplex™ SARS-CoV-2 Variants I Assay kit in identifying mutations in the variants.

Study design

. We retrospectively examined 30 stored nasal swa

SUBMITTER: Kami W 

PROVIDER: S-EPMC8164504 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature