Intraoperative visualization of nerves using a myelin protein-zero specific fluorescent tracer.

ABSTRACT:

Background

Surgically induced nerve damage is a common but debilitating side effect in oncological surgery. With the aim to use fluorescence guidance to enable nerve-sparing interventions in future surgery, a fluorescent tracer was developed that specifically targets myelin protein zero (P0).

Results

Truncated homotypic P0 protein-based peptide sequences were C-terminally functionalized with the far-red cyanine dye Cy5. The lead compound Cy5-P0_101-125 was selected after initial solubility, (photo)physical and in vitro evaluation (including P0-blocking experiments). Cy5-P0_101-125 (K_D = 105 ± 17 nM) allowed in vitro and ex vivo P0-related staining. Furthermore, Cy5-P0_101-125  enabled in vivo fluorescence imaging of the Sciatic nerve

SUBMITTER: Buckle T 

PROVIDER: S-EPMC8164657 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature