Mvda is required for zebrafish early development.

ABSTRACT:

Background

The MVD gene mutations are identified in porokeratosis, which is considered a skin-specific autoinflammatory keratinization disease. However, the biological function of MVD gene remains largely unknown. Therefore, we analyzed the function of mvda gene, orthologous to the human MVD gene, in developing zebrafish.

Methods

Morpholino antisense oligonucleotide technique was used to generate mvda loss-of-function phenotypes. Knockdown of mvda was confirmed by RT-PCR and Sanger sequencing. Scanning and transmission electron microscopy were performed to analyze the morphology of the epidermis. Angiogenesis study was presented using the Tg(fli1a:EGFP)^y1 transgenic strain. In addition, acridine orange staining was used to examine the apoptotic cells in vivo.

R

SUBMITTER: Wong W 

PROVIDER: S-EPMC8164810 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature