Identifying miRNAs in multiple sclerosis gray matter lesions that correlate with atrophy measures.

ABSTRACT:

Objective

Multiple sclerosis (MS) is an inflammatory, demyelinating and neurodegenerative disease of the central nervous system (CNS). Though MS was initially considered to be a white matter demyelinating disease, myelin loss in cortical gray matter has been reported in all disease stages. We previously identified microRNAs (miRNAs) in white matter lesions (WMLs) that are detected in serum from MS patients. However, miRNA expression profiles in gray matter lesions (GMLs) from progressive MS brains are understudied.

Methods

We used a combination of global miRNAs and gene expression profiling of GMLs and independent validation using real-time quantitative polymerase chain reaction (RT-qPCR), immuno-in situ hybridization, and immunohistochemistry.

Results

Compared to mat

SUBMITTER: Tripathi A 

PROVIDER: S-EPMC8164853 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature