The mitochondrial NADH pool is involved in hydrogen sulfide signaling and stimulation of aerobic glycolysis.

ABSTRACT: Hydrogen sulfide is synthesized by enzymes involved in sulfur metabolism and oxidized via a dedicated mitochondrial pathway that intersects with the electron transport chain at the level of complex III. Studies with H₂S are challenging since it is volatile and also reacts with oxidized thiols in the culture medium, forming sulfane sulfur species. The half-life of exogenously added H₂S to cultured cells is unknown. In this study, we first examined the half-life of exogenously added H₂S to human colonic epithelial cells. In plate cultures, H₂S disappeared with a t¹/₂ of 3 to 4 min at 37 °C with a small fraction being trapped as sulfane sulfur species. In suspension cultures, the rate of abiotic loss of H₂S was slowe

SUBMITTER: Vitvitsky V 

PROVIDER: S-EPMC8165552 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature