An integrated pipeline for high-throughput screening and profiling of spheroids using simple live image analysis of frame to frame variations.

ABSTRACT: High-throughput imaging methods can be applied to relevant cell culture models, fostering their use in research and translational applications. Improvements in microscopy, computational capabilities and data analysis have enabled high-throughput, high-content approaches from endpoint 2D microscopy images. Nonetheless, trade-offs in acquisition, computation and storage between content and throughput remain, in particular when cells and cell structures are imaged in 3D. Moreover, live 3D phase contrast microscopy images are not often amenable to analysis because of the high level of background noise. Cultures of Human induced pluripotent stem cells (hiPSC) offer unprecedented scope to profile and screen conditions affecting cell fate decisions, self-organisation and early embryonic developme

SUBMITTER: Alsehli H 

PROVIDER: S-EPMC8165939 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature