Antisense yycG modulates the susceptibility of Staphylococcus aureus to hydrogen peroxide via the sarA.

ABSTRACT:

Background

The infectious pathogen Staphylococcus aureus (S. aureus) is primarily associated with osteomyelitis. Hydrogen peroxide drainage is an effective antimicrobial treatment that has been adopted to combat S. aureus infections. Previous investigations have indicated that the antisense RNA (asRNA) strategy negatively modulates S. aureus YycFG TCS, and it significantly disrupts biofilm formation. However, the effects of the antisense yycG RNA (ASyycG) strategy on the susceptibility of biofilm-producing S. aureus to hydrogen peroxide and the mechanisms underlying this effect have not been elucidated to date.

Results

Overexpression of ASyycG inhibited the transcription of biofilm formation-related genes, including sarA and icaA. Additionally, the CFU counts and the live ba

SUBMITTER: Wu S 

PROVIDER: S-EPMC8165985 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature