Targeting multiple genes containing long mononucleotide A-T repeats in lung cancer stem cells.

ABSTRACT:

Background

Intratumour heterogeneous gene expression among cancer and cancer stem cells (CSCs) can cause failure of current targeted therapies because each drug aims to target the function of a single gene. Long mononucleotide A-T repeats are cis-regulatory transcriptional elements that control many genes, increasing the expression of numerous genes in various cancers, including lung cancer. Therefore, targeting A-T repeats may dysregulate many genes driving cancer development. Here, we tested a peptide nucleic acid (PNA) oligo containing a long A-repeat sequence [A(15)] to disrupt the transcriptional control of the A-T repeat in lung cancer and CSCs.

Methods

First, we separated CSCs from parental lung cancer cell lines. Then, we evaluated the role of A-T repeat gene regulat

SUBMITTER: Bhummaphan N 

PROVIDER: S-EPMC8166091 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature