Network-Related Changes in Neurotransmitters and Seizure Propagation During Rodent Epileptogenesis.

ABSTRACT:

Objective

To test the hypothesis that glutamate and GABA are linked to the formation of epilepsy networks and the triggering of spontaneous seizures, we examined seizure initiation/propagation characteristics and neurotransmitter levels during epileptogenesis in a translationally relevant rodent model of mesial temporal lobe epilepsy.

Methods

The glutamine synthetase (GS) inhibitor methionine sulfoximine was infused into one of the hippocampi in laboratory rats to create a seizure focus. Long-term video-intracranial EEG recordings and brain microdialysis combined with mass spectrometry were used to examine seizure initiation, seizure propagation, and extracellular brain levels of glutamate and GABA.

Results

All seizures (n = 78 seizures, n = 3 rats) appeared first in

SUBMITTER: Dhaher R 

PROVIDER: S-EPMC8166437 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature