Ontology highlight
ABSTRACT:
SUBMITTER: Kim A
PROVIDER: S-EPMC8166669 | biostudies-literature | 2021 Jun
REPOSITORIES: biostudies-literature
Kim Annie A Lee Kun-Gu KG Kwon Yeongbeen Y Lee Kang-In KI Yang Heung-Mo HM Habib Omer O Kim Jihun J Kim Sang-Tae ST Kim Sung Joo SJ Kim Jin-Soo JS Hwang Dong-Youn DY
Stem cell reviews and reports 20210109 3
Human embryonic stem cells (hESCs) hold promise in regenerative medicine but allogeneic immune rejections caused by highly polymorphic human leukocyte antigens (HLAs) remain a barrier to their clinical applications. Here, we used a CRISPR/Cas9-mediated HLA-editing strategy to generate a variety of HLA homozygous-like hESC lines from pre-established hESC lines. We edited four pre-established HLA-heterozygous hESC lines and created a mini library of 14 HLA-edited hESC lines in which single HLA-A a ...[more]