Subcellular spatio-temporal intravital kinetics of aflatoxin B₁ and ochratoxin A in liver and kidney.

ABSTRACT: Local accumulation of xenobiotics in human and animal tissues may cause adverse effects. Large differences in their concentrations may exist between individual cell types, often due to the expression of specific uptake and export carriers. Here we established a two-photon microscopy-based technique for spatio-temporal detection of the distribution of mycotoxins in intact kidneys and livers of anesthetized mice with subcellular resolution. The mycotoxins ochratoxin A (OTA, 10 mg/kg b.w.) and aflatoxin B₁ (AFB₁, 1.5 mg/kg b.w.), which both show blue auto-fluorescence, were analyzed after intravenous bolus injections. Within seconds after administration, OTA was filtered by glomeruli, and enriched in distal tubular epithelial cells (dTEC). A striking feature of AFB₁

SUBMITTER: Ghallab A 

PROVIDER: S-EPMC8166722 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature