Influence of the densities and nutritional components of bacterial colonies on the culture-enriched gut bacterial community structure.

ABSTRACT: Isolating relevant microorganisms is still a substantial challenge that limits the use of bacteria in the maintenance of human health. To confirm which media and which bacterial colony densities can enrich certain kinds of bacteria, we selected eight common media and used them to enrich the gut microorganisms on agar plates. Then, we calculated the numbers of bacterial colonies and collected the bacterial culture mixtures from each kind of medium. Using the Illumina HiSeq platform, we analyzed the composition and diversity of the culture-enriched gut bacterial community. Our data suggested that medium supplemented with blood could increase the diversity of the bacterial community. In addition, beef powder and peptone could significantly change the culture-enriched bacterial community. A mo

SUBMITTER: Gu Y 

PROVIDER: S-EPMC8167003 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature