Cryo-EM Map-Based Model Validation Using the False Discovery Rate Approach.

ABSTRACT: Significant technological developments and increasing scientific interest in cryogenic electron microscopy (cryo-EM) has resulted in a rapid increase in the amount of data generated by these experiments and the derived atomic models. Robust measures for the validation of 3D reconstructions and atomic models are essential for appropriate interpretation of the data. The resolution of data and availability of software tools that work across a range of resolutions often limit the quality of derived models. Hence, the final atomic model is often incomplete or contains regions where atomic positions are less reliable or incorrectly built. Extensive manual pruning and local adjustments or rebuilding are usually required to address these issues. The presented research introduces a software tool fo

SUBMITTER: Olek M 

PROVIDER: S-EPMC8167059 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature