Transient incubation of cultured hippocampal neurons in the absence of magnesium induces rhythmic and synchronized epileptiform-like activity.

ABSTRACT: Cell culture models are important tools to study epileptogenesis mechanisms. The aim of this work was to characterize the spontaneous and synchronized rhythmic activity developed by cultured hippocampal neurons after transient incubation in zero Mg²⁺ to model Status Epilepticus. Cultured hippocampal neurons were transiently incubated with a Mg²⁺-free solution and the activity of neuronal networks was evaluated using single cell calcium imaging and whole-cell current clamp recordings. Here we report the development of synchronized and spontaneous [Ca²⁺]_i transients in cultured hippocampal neurons immediately after transient incubation in a Mg²⁺-free solution. Spontaneous and synchronous [Ca²⁺]_i oscillations were ob

SUBMITTER: Mele M 

PROVIDER: S-EPMC8167095 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature