Dataset Information

Expression of leukotriene B₄ receptor 1 defines functionally distinct DCs that control allergic skin inflammation.

ABSTRACT: Leukotriene B₄ (LTB₄) receptor 1 (BLT1) is a chemotactic G protein-coupled receptor expressed by leukocytes, such as granulocytes, macrophages, and activated T cells. Although there is growing evidence that BLT1 plays crucial roles in immune responses, its role in dendritic cells remains largely unknown. Here, we identified novel DC subsets defined by the expression of BLT1, namely, BLT1^hi and BLT1^lo DCs. We also found that BLT1^hi and BLT1^lo DCs differentially migrated toward LTB₄ and CCL21, a lymph node-homing chemoattractant, respectively. By generating LTB₄-producing enzyme LTA₄H knockout mice and CD11c promoter-driven Cre recombinase-expressing BLT1 conditional knockout (BLT1 cKO) mice, we showed that the migration of BLT1^hi DCs exacerbated allergic contact dermatitis. Comprehensive transcriptome analysis revealed that BLT1^hi DCs preferentially induced Th1 differentiation by upregulating IL-12p35 expression, whereas BLT1^lo DCs accelerated T cell proliferation by producing IL-2. Collectively, the data reveal an unexpected role for BLT1 as a novel DC subset marker and provide novel insights into the role of the LTB₄-BLT1 axis in the spatiotemporal regulation of distinct DC subsets.

SUBMITTER: Koga T

PROVIDER: S-EPMC8167169 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Expression of leukotriene B4 receptor 1 defines functionally distinct DCs that control allergic skin inflammation.

Koga Tomoaki T Sasaki Fumiyuki F Saeki Kazuko K Tsuchiya Soken S Okuno Toshiaki T Ohba Mai M Ichiki Takako T Iwamoto Satoshi S Uzawa Hirotsugu H Kitajima Keiko K Meno Chikara C Nakamura Eri E Tada Norihiro N Fukui Yoshinori Y Kikuta Junichi J Ishii Masaru M Sugimoto Yukihiko Y Nakao Mitsuyoshi M Yokomizo Takehiko T

Cellular & molecular immunology 20201009 6

Leukotriene B4 (LTB4) receptor 1 (BLT1) is a chemotactic G protein-coupled receptor expressed by leukocytes, such as granulocytes, macrophages, and activated T cells. Although there is growing evidence that BLT1 plays crucial roles in immune responses, its role in dendritic cells remains largely unknown. Here, we identified novel DC subsets defined by the expression of BLT1, namely, BLT1hi and BLT1lo DCs. We also found that BLT1hi and BLT1<s ...[more]

PMID: 33037399

Similar Datasets

Project description:Multi-walled carbon nanotubes (MWCNT) have been reported to promote lung inflammation and fibrosis. The commercial demand for nanoparticle-based materials has expanded rapidly and as demand for nanomaterials grows, so does the urgency of establishing an appreciation of the degree of health risk associated with their increased production and exposure. In this study, we examined whether MWCNT inhalation elicited pulmonary eosinophilic inflammation and influenced the development of allergic airway inflammatory responses. Our data revealed that instillation of FA21 MWCNT into the airways of mice resulted in a rapid increase, within 24 h, in the number of eosinophils present in the lungs. The inflammatory response elicited was also associated with an increase in the level of cysteinyl leukotrienes (cysLTs) present in the bronchoalveolar lavage fluid. CysLTs were implicated in the airway inflammatory response since pharmacological inhibition of their biosynthesis using the 5-lipoxygenase inhibitor Zileuton resulted in a marked reduction in the severity of inflammation observed. Moreover, FA21 MWCNT entering the airways of mice suffering from house dust mite (HDM)-elicited allergic lung inflammation markedly exacerbated the intensity of the airway inflammation. This response was characterized by a pulmonary eosinophilia, lymphocyte infiltration, and raised cysLT levels. The severity of pulmonary inflammation caused by either inhalation of MWCNT alone or in conjunction with HDM allergen correlated with the level of nickel present in the material, since preparations that contained higher levels of nickel (FA21, 5.54% Ni by weight) were extremely effective at eliciting or exacerbating inflammatory or allergic responses while preparations containing lower amounts of nickel (FA04, 2.54% Ni by weight) failed to initiate or exacerbate pulmonary inflammation. In summary, instillation of high nickel MWCNT into the lungs promoted eosinophilic inflammation and caused an intense exacerbation of pre-existing allergic airway inflammation by facilitating cysLT biosynthesis. These findings suggest that exposure to airborne MWCNT is likely to have adverse inflammatory effects in individuals suffering from atopic asthma and, in this context, further investigation of the therapeutic effects of pharmacological agents that block leukotriene synthesis is warranted.

Dataset Information

Expression of leukotriene B₄ receptor 1 defines functionally distinct DCs that control allergic skin inflammation.

Publications

Expression of leukotriene B<sub>4</sub> receptor 1 defines functionally distinct DCs that control allergic skin inflammation.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets