First report of tilapia lake virus emergence in fish farms in the department of Cordoba, Colombia.

ABSTRACT:

Background and aim

In 2016, the tilapia-producing farms in the department of Córdoba, Colombia, had witnessed outbreaks of disease with clinical signs compatible with those caused by the tilapia lake virus (TiLV). This study was conducted to confirm the presence of TiLV in some fish farms in the department of Córdoba.

Materials and methods

A descriptive cross-sectional study was conducted in seven farms using a non-random sampling method from July 2016 to December 2017. A total of 66 fish, including 33 healthy fish and 33 fish with clinical signs, were caught, from which 178 tissue samples of spleen, liver, and brain were collected. RNA was extracted from each organ using TRIzol^®. cDNA was synthesized using a retrotranscriptase and a universal amplification primer

SUBMITTER: Contreras H 

PROVIDER: S-EPMC8167540 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature