Histone Sample Preparation for Bottom-Up Mass Spectrometry: A Roadmap to Informed Decisions.

ABSTRACT: Histone-based chromatin organization enabled eukaryotic genome complexity. This epigenetic control mechanism allowed for the differentiation of stable gene-expression and thus the very existence of multicellular organisms. This existential role in biology makes histones one of the most complexly modified molecules in the biotic world, which makes these key regulators notoriously hard to analyze. We here provide a roadmap to enable fast and informed selection of a bottom-up mass spectrometry sample preparation protocol that matches a specific research question. We therefore propose a two-step assessment procedure: (i) visualization of the coverage that is attained for a given workflow and (ii) direct alignment between runs to assess potential pitfalls at the ion level. To illustrate the app

SUBMITTER: Daled S 

PROVIDER: S-EPMC8167631 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature