Apolipoprotein M promotes cholesterol uptake and efflux from mouse macrophages.

ABSTRACT: Apolipoprotein M (ApoM) exhibits various anti-atherosclerotic functions as a component of high-density lipoprotein (HDL) particles. Scavenger receptor class B type I (SR-BI) is a classic HDL receptor that mediates selective cholesterol uptake and enhances the efflux of cellular cholesterol to HDL. However, the effect of ApoM on cholesterol transport in macrophages remains unclear. In this study, we identified for the first time that ApoM is expressed in mouse macrophages and is involved in cholesterol uptake, similar to SR-BI. NBD-cholesterol uptake and efflux in cells were characterized using fluorescence spectrophotometry. The uptake ratios of cholesterol by macrophages from ApoM^-/- SR-BI^-/- mice were significantly lower than those from ApoM^+/+ SR-BI

SUBMITTER: Yao S 

PROVIDER: S-EPMC8167864 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature