ESCRT, not intralumenal fragments, sorts ubiquitinated vacuole membrane proteins for degradation.

ABSTRACT: The lysosome (or vacuole in fungi and plants) is an essential organelle for nutrient sensing and cellular homeostasis. In response to environmental stresses such as starvation, the yeast vacuole can adjust its membrane composition by selectively internalizing membrane proteins into the lumen for degradation. Regarding the selective internalization mechanism, two competing models have been proposed. One model suggests that the ESCRT machinery is responsible for the sorting. In contrast, the ESCRT-independent intralumenal fragment (ILF) pathway proposes that the fragment generated by homotypic vacuole fusion is responsible for the sorting. Here, we applied a microfluidics-based imaging method to capture the complete degradation process in vivo. Combining live-cell imaging with a synchronized

SUBMITTER: Yang X 

PROVIDER: S-EPMC8167898 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature