Carbon monoxide alleviates senescence in diabetic nephropathy by improving autophagy.

ABSTRACT:

Objectives

Senescence, characterized by permanent cycle arrest, plays an important role in diabetic nephropathy (DN). However, the mechanism of renal senescence is still unclear, and the treatment targeting it remains to be further explored.

Materials and methods

The DN mice were induced by HFD and STZ, and 3 types of renal cells were treated with high glucose (HG) to establish in vitro model. Senescence-related and autophagy-related markers were detected by qRT-PCR and Western blot. Further, autophagy inhibitors and co-immunoprecipitation were used to clarify the mechanism of CO. Additionally, the specific relationship between autophagy and senescence was explored by immunofluorescence triple co-localization and ELISA.

Results

We unravelled that senescence occurred i

SUBMITTER: Chen L 

PROVIDER: S-EPMC8168421 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature