Natural antisense transcript of Period2, Per2AS, regulates the amplitude of the mouse circadian clock.

ABSTRACT: In mammals, a set of core clock genes form transcription-translation feedback loops to generate circadian oscillations. We and others recently identified a novel transcript at the Period2 (Per2) locus that is transcribed from the antisense strand of Per2 This transcript, Per2AS, is expressed rhythmically and antiphasic to Per2 mRNA, leading to our hypothesis that Per2AS and Per2 mutually inhibit each other's expression and form a double negative feedback loop. By perturbing the expression of Per2AS, we found that Per2AS transcription, but not transcript, represses Per2 However, Per2 does not repress Per2AS, as Per2 knockdown led to a decrease in the Per2AS level, indicating that Per2AS

SUBMITTER: Mosig RA 

PROVIDER: S-EPMC8168560 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature