Comparative proteomic analysis of dental cementum from deciduous and permanent teeth.

ABSTRACT:

Background and objectives

Dental cementum (DC) is a mineralized tissue covering tooth roots that plays a critical role in dental attachment. Differences in deciduous vs. permanent tooth DC have not been explored. We hypothesized that proteomic analysis of DC matrix would identify compositional differences in deciduous (DecDC) vs. permanent (PermDC) cementum that might reflect physiological or pathological differences, such as root resorption that is physiological in deciduous teeth but can be pathological in the permanent dentition.

Methods

Protein extracts from deciduous (n = 25) and permanent (n = 12) teeth were pooled (five pools of DecDC, five teeth each; four pools of PermDC, three teeth each). Samples were denatured, and proteins were extracted, reduced, alkylated, dig

SUBMITTER: Giovani PA 

PROVIDER: S-EPMC8168683 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature