Efficiency comparison of B6(Cg)-Tyr^c-2j /J and C57BL/6NTac embryos as hosts for the generation of knockout mice.

ABSTRACT: Careful selection of the host embryo is critical to the efficient production of knockout (KO) mice when injecting mouse embryonic stem (mES) cells into blastocysts. B6(Cg)-Tyr^c-2j/J (B6 albino) and C57BL/6NTac (B6NTac) strains of mice are widely used to produce host blastocysts for such procedures. Here, we tested these two strains to identify an appropriate match for modified agouti C57BL/6N (JM8A3.N1) mES cells. When comparing blastocyst yield, super-ovulated B6NTac mice produced more injectable blastocysts per female than B6 albino mice (8.2 vs. 5.4). There was no significant difference in birth rate when injected embryos were transferred to the same pseudopregnant recipient strain. However, the live birth rate was significantly higher for B6NTac blastocysts than B6 albino bl

SUBMITTER: Ma Y 

PROVIDER: S-EPMC8169498 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature