Early loss of cerebellar Purkinje cells in human and a transgenic mouse model of Alzheimer's disease.

ABSTRACT:

Background

The cerebellum's involvement in AD has been under-appreciated by historically labeling as a normal control in AD research.

Methods

We determined the involvement of the cerebellum in AD progression. Postmortem human and APPswe/PSEN1dE9 mice cerebellums were used to assess the cerebellar Purkinje cells (PC) by immunohistochemistry. The locomotor and spatial cognitive functions were assessed in 4- to 5-month-old APPswe/PSEN1dE9 mice. Aβ plaque and APP processing were determined in APPswe/PSEN1dE9 mice at different age groups by immunohistochemistry and Western blot.

Results

We observed loss of cerebellar PC in mild cognitive impairment and AD patients compared with cognitively normal controls. A strong trend towards PC loss was found in AD mice as early as 5 m

SUBMITTER: Chaudhari K 

PROVIDER: S-EPMC8169538 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature