Growth and Differentiation of Circulating Stem Cells After Extensive Ex Vivo Expansion.

ABSTRACT:

Background

Stem cell therapy is gaining momentum as an effective treatment strategy for degenerative diseases. Adult stem cells isolated from various sources (i.e., cord blood, bone marrow, adipose tissue) are being considered as a realistic option due to their well-documented therapeutic potentials. Our previous studies standardized a method to isolate circulating multipotent cells (CMCs) that are able to sustain long term in vitro culture and differentiate towards mesodermal lineages.

Methods

In this work, long-term cultures of CMCs were stimulated to study in vitro neuronal and myogenic differentiation. After induction, cells were analysed at different time points. Morphological studies were performed by scanning electron microscopy and specific neuronal and myogenic mark

SUBMITTER: Barbon S 

PROVIDER: S-EPMC8169750 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature