Quantifying cell cycle-dependent drug sensitivities in cancer using a high throughput synchronisation and screening approach.

ABSTRACT:

Background

Chemotherapy and targeted agent anti-cancer efficacy is largely dependent on the proliferative state of tumours, as exemplified by agents that target DNA synthesis/replication or mitosis. As a result, cell cycle specificities of a number of cancer drugs are well known. However, they are yet to be described in a quantifiable manner.

Methods

A scalable cell synchronisation protocol used to screen a library of 235 anti-cancer compounds exposed over six hours in G1 or S/G2 accumulated AsPC-1 cells to generate a cell cycle specificity (CCS) score.

Findings

The synchronisation method was associated with reduced method-related cytotoxicity compared to nocodazole, delivering sufficient cell cycle purity and cell numbers to run high-throughput drug library screens.

SUBMITTER: Johnson TI 

PROVIDER: S-EPMC8170111 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature