C1qa deficiency in mice increases susceptibility to mouse hepatitis virus A59 infection.

ABSTRACT:

Background

Mouse hepatitis virus (MHV) A59 is a highly infectious pathogen and starts in the respiratory tract and progresses to systemic infection in laboratory mice. The complement system is an important part of the host immune response to viral infection. It is not clear the role of the classical complement pathway in MHV infection.

Objectives

The purpose of this study was to determine the importance of the classical pathway in coronavirus pathogenesis by comparing C1qa KO mice and wild-type mice.

Methods

We generated a C1qa KO mouse using CRISPR/Cas9 technology and compared the susceptibility to MHV A59 infection between C1qa KO and wild-type mice. Histopathological and immunohistochemical changes, viral loads, and chemokine expressions in bot

SUBMITTER: Kim HW 

PROVIDER: S-EPMC8170211 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature