Resource-efficient internally controlled in-house real-time PCR detection of SARS-CoV-2.

ABSTRACT:

Background

The reliable detection of SARS-CoV-2 has become one of the most important contributions to COVID-19 crisis management. With the publication of the first sequences of SARS-CoV-2, several diagnostic PCR assays have been developed and published. In addition to in-house assays the market was flooded with numerous commercially available ready-to-use PCR kits, with both approaches showing alarming shortages in reagent supply.

Aim

Here we present a resource-efficient in-house protocol for the PCR detection of SARS-CoV-2 RNA in patient specimens (RKI/ZBS1 SARS-CoV-2 protocol).

Methods

Two duplex one-step real-time RT-PCR assays are run simultaneously and provide information on two different SARS-CoV-2 genomic regions. Each one is duplexed with a control that either

SUBMITTER: Michel J 

PROVIDER: S-EPMC8170437 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature