Screening of potential vaccine candidates against pathogenic Brucella spp. using compositive reverse vaccinology.

ABSTRACT: Brucella spp. are Gram-negative, facultative intracellular bacteria that cause brucellosis in humans and various animals. The threat of brucellosis has increased, yet currently available live attenuated vaccines still have drawbacks. Therefore, subunit vaccines, produced using protein antigens and having the advantage of being safe, cost-effective and efficacious, are urgently needed. In this study, we used core proteome analysis and a compositive RV methodology to screen potential broad-spectrum antigens against 213 pathogenic strains of Brucella spp. with worldwide geographic distribution. Candidate proteins were scored according to six biological features: subcellular localization, antigen similarity, antigenicity, mature epitope density, virulence, and adhesion probability. In the RV a

SUBMITTER: Zai X 

PROVIDER: S-EPMC8170439 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature