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High-resolution analysis of chromosome conformation in hyperthermophilic archaea.

ABSTRACT: Chromosome conformation capture (3C) techniques are emerging as promising approaches to study genome organization in Archaea, the least understood domain of life in terms of chromosome biology. Here, we describe a 3C technique combined with deep sequencing for the hyperthermophilic archaeal genus Sulfolobus. Instead of using restriction enzymes compatible with fill-in labeling, this protocol uses the 4-bp blunt cutter AluI to generate high-resolution (up to 2 kb) contact maps from Sulfolobus species. For complete details on the use and execution of this protocol, please refer to Takemata and Bell (2021).

SUBMITTER: Takemata N

PROVIDER: S-EPMC8170502 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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High-resolution analysis of chromosome conformation in hyperthermophilic archaea.

Takemata Naomichi N Bell Stephen D SD

STAR protocols 20210528 2

Chromosome conformation capture (3C) techniques are emerging as promising approaches to study genome organization in Archaea, the least understood domain of life in terms of chromosome biology. Here, we describe a 3C technique combined with deep sequencing for the hyperthermophilic archaeal genus Sulfolobus. Instead of using restriction enzymes compatible with fill-in labeling, this protocol uses the 4-bp blunt cutter AluI to generate high-resolution (up to 2 kb) contact maps from Sulf ...[more]

PMID: 34113850

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