A survey of the mouse hindbrain in the fed and fasted states using single-nucleus RNA sequencing.

ABSTRACT:

Objective

The area postrema (AP) and nucleus tractus solitarius (NTS) located in the hindbrain are key nuclei that sense and integrate peripheral nutritional signals and consequently regulate feeding behaviour. While single-cell transcriptomics have been used in mice to reveal the gene expression profile and heterogeneity of key hypothalamic populations, similar in-depth studies have not yet been performed in the hindbrain.

Methods

Using single-nucleus RNA sequencing, we provide a detailed survey of 16,034 cells within the AP and NTS of mice in the fed and fasted states.

Results

Of these, 8,910 were neurons that group into 30 clusters, with 4,289 from mice fed ad libitum and 4,621 from overnight fasted mice. A total of 7,124 nuclei were from non-neuronal cells, includ

SUBMITTER: Dowsett GKC 

PROVIDER: S-EPMC8170503 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature