Clinical Evolution of Epithelial-Mesenchymal Transition in Human Carcinomas.

ABSTRACT: The significance of the phenotypic plasticity afforded by epithelial-mesenchymal transition (EMT) for cancer progression and drug resistance remains to be fully elucidated in the clinic. We evaluated epithelial-mesenchymal phenotypic characteristics across a range of tumor histologies using a validated, high-resolution digital microscopic immunofluorescence assay (IFA) that incorporates β-catenin detection and cellular morphology to delineate carcinoma cells from stromal fibroblasts and that quantitates the individual and colocalized expression of the epithelial marker E-cadherin (E) and the mesenchymal marker vimentin (V) at subcellular resolution ("EMT-IFA"). We report the discovery of β-catenin⁺ cancer cells that coexpress E-cadherin and vimentin in core-needle biopsies from

SUBMITTER: Navas T 

PROVIDER: S-EPMC8170833 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature