Rapid and simple single-chamber nucleic acid detection system prepared through nature-inspired surface engineering.

ABSTRACT: Background: Nucleic acid (NA)-based diagnostics enable a rapid response to various diseases, but current techniques often require multiple labor-intensive steps, which is a major obstacle to successful translation to a clinical setting. Methods: We report on a surface-engineered single-chamber device for NA extraction and in situ amplification without sample transfer. Our system has two reaction sites: a NA extraction chamber whose surface is patterned with micropillars and a reaction chamber filled with reagents for in situ polymerase-based NA amplification. These two sites are integrated in a single microfluidic device; we applied plastic injection molding for cost-effective, mass-production of the designed device. The micropillars were chemically activated vi

SUBMITTER: Park J 

PROVIDER: S-EPMC8171086 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature