Differential methylation analysis for bisulfite sequencing using DSS.

ABSTRACT: Bisulfite sequencing (BS-seq) technology measures DNA methylation at single nucleotide resolution. A key task in BS-seq data analysis is to identify differentially methylation (DM) under different conditions. Here we provide a tutorial for BS-seq DM analysis using Bioconductor package DSS. DSS uses a beta-binomial model to characterize the sequence counts from BS-seq, and implements rigorous statistical method for hypothesis testing. It provides flexible functionalities for a variety of DM analyses.

SUBMITTER: Feng H 

PROVIDER: S-EPMC8171293 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature