Motor endplate-expressing cartilage-muscle implants for reconstruction of a denervated hemilarynx.

ABSTRACT:

Objective

Tissue engineering of the larynx requires a complex, multiple tissue layer design. Additionally, spontaneous reinnervation of the larynx after recurrent laryngeal nerve (RLN) injury is often disorganized, resulting in subpar function. This study investigates use of tissue-engineered cartilage and motor endplate-expressing (MEE) tissue-engineered skeletal muscle implants for laryngeal reconstruction and the promotion of organized reinnervation after RLN injury.

Methods

F344 rat primary muscle progenitor cells (MPCs) were isolated. Three-dimensional muscle constructs were created by encapsulating MPCs in type I oligomeric collagen under passive tension. Constructs were then cultured in differentiation medium (MPC control constructs) or induced to form motor endplates

SUBMITTER: Brookes S 

PROVIDER: S-EPMC8171591 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature