A method for achieving complete microbial genomes and improving bins from metagenomics data.

ABSTRACT: Metagenomics facilitates the study of the genetic information from uncultured microbes and complex microbial communities. Assembling complete genomes from metagenomics data is difficult because most samples have high organismal complexity and strain diversity. Some studies have attempted to extract complete bacterial, archaeal, and viral genomes and often focus on species with circular genomes so they can help confirm completeness with circularity. However, less than 100 circularized bacterial and archaeal genomes have been assembled and published from metagenomics data despite the thousands of datasets that are available. Circularized genomes are important for (1) building a reference collection as scaffolds for future assemblies, (2) providing complete gene content of a genome, (3) confi

SUBMITTER: Lui LM 

PROVIDER: S-EPMC8172020 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature