PrintrLab incubator: A portable and low-cost CO2 incubator based on an open-source 3D printer architecture.

ABSTRACT: Growth in open-source hardware designs combined with the decreasing cost of high-quality 3D printers have supported a resurgence of in-house custom lab equipment development. Herein, we describe a low-cost (< $400), open-source CO2 incubator. The system is comprised of a Raspberry Pi computer connected to a 3D printer controller board that has controls for a CO2 sensor, solenoid valve, heater, and thermistors. CO2 is supplied through the sublimation of dry ice stored inside a thermos to create a sustained 5% CO2 supply. The unit is controlled via G-Code commands sent by the Raspberry Pi to the controller board. In addition, we built a custom software application for remote control and used the open-source Grafana dashboard for remote monitoring. Our data show that we can maintain consisten

SUBMITTER: Arumugam A 

PROVIDER: S-EPMC8172042 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature