Rapid fabrication of hydrogel micropatterns by projection stereolithography for studying self-organized developmental patterning.

ABSTRACT: Self-organized patterning of mammalian embryonic stem cells on micropatterned surfaces has previously been established as an in vitro platform for early mammalian developmental studies, complimentary to in vivo studies. Traditional micropatterning methods, such as micro-contact printing (μCP), involve relatively complicated fabrication procedures, which restricts widespread adoption by biologists. Here, we demonstrate a rapid method of micropatterning by printing hydrogel micro-features onto a glass-bottomed culture vessel. The micro-features are printed using a projection stereolithography bioprinter yielding hydrogel structures that geometrically restrict the attachment of cells or proteins. Compared to traditional and physical photomasks, a digitally tunable virtual photomask is used in

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC8172057 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature