Comparative tRNA sequencing and RNA mass spectrometry for surveying tRNA modifications.

ABSTRACT: Chemical modifications of the nucleosides that comprise transfer RNAs are diverse. However, the structure, location and extent of modifications have been systematically charted in very few organisms. Here, we describe an approach in which rapid prediction of modified sites through reverse transcription-derived signatures in high-throughput transfer RNA-sequencing (tRNA-seq) data is coupled with identification of tRNA modifications through RNA mass spectrometry. Comparative tRNA-seq enabled prediction of several Vibrio cholerae modifications that are absent from Escherichia coli and also revealed the effects of various environmental conditions on V. cholerae tRNA modification. Through RNA mass spectrometric analyses, we showed that two of the V. cholerae-specific reverse transcription signa

SUBMITTER: Kimura S 

PROVIDER: S-EPMC8172280 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature