Construction of a mammalian embryo model from stem cells organized by a morphogen signalling centre.

ABSTRACT: Generating properly differentiated embryonic structures in vitro from pluripotent stem cells remains a challenge. Here we show that instruction of aggregates of mouse embryonic stem cells with an experimentally engineered morphogen signalling centre, that functions as an organizer, results in the development of embryo-like entities (embryoids). In situ hybridization, immunolabelling, cell tracking and transcriptomic analyses show that these embryoids form the three germ layers through a gastrulation process and that they exhibit a wide range of developmental structures, highly similar to neurula-stage mouse embryos. Embryoids are organized around an axial chordamesoderm, with a dorsal neural plate that displays histological properties similar to the murine embryo neuroepithelium and that f

SUBMITTER: Xu PF 

PROVIDER: S-EPMC8172561 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature