Development of a loop-mediated isothermal amplification assay for the detection of Tilletia controversa based on genome comparison.

ABSTRACT: Tilletia controversa causing dwarf bunt of wheat is a quarantine pathogen in several countries. Therefore, its specific detection is of great phytosanitary importance. Genomic regions routinely used for phylogenetic inferences lack suitable polymorphisms for the development of species-specific markers. We therefore compared 21 genomes of six Tilletia species to identify DNA regions that were unique and conserved in all T. controversa isolates and had no or limited homology to other Tilletia species. A loop-mediated isothermal amplification (LAMP) assay for T. controversa was developed based on one of these DNA regions. The specificity of the assay was verified using 223 fungal samples comprising 43 fungal species including 11 Tilletia species, in particular 39 specimens of T. controversa,

SUBMITTER: Sedaghatjoo S 

PROVIDER: S-EPMC8172862 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature