Detection of SARS-CoV-2 RNA using RT-LAMP and molecular beacons.

ABSTRACT:

Background

Rapid spread of SARS-CoV-2 has led to a global pandemic, resulting in the need for rapid assays to allow diagnosis and prevention of transmission. Reverse transcription-polymerase chain reaction (RT-PCR) provides a gold standard assay for SARS-CoV-2 RNA, but instrument costs are high and supply chains are potentially fragile, motivating interest in additional assay methods. Reverse transcription and loop-mediated isothermal amplification (RT-LAMP) provides an alternative that uses orthogonal and often less expensive reagents without the need for thermocyclers. The presence of SARS-CoV-2 RNA is typically detected using dyes to report bulk amplification of DNA; however, a common artifact is nonspecific DNA amplification, which complicates detection.

Results

Here we

SUBMITTER: Sherrill-Mix S 

PROVIDER: S-EPMC8173101 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature