Dataset Information

Soybean Inoculated With One Bradyrhizobium Strain Isolated at Elevated [CO₂] Show an Impaired C and N Metabolism When Grown at Ambient [CO₂].

ABSTRACT: Soybean (Glycine max L.) future response to elevated [CO₂] has been shown to differ when inoculated with B. japonicum strains isolated at ambient or elevated [CO₂]. Plants, inoculated with three Bradyrhizobium strains isolated at different [CO₂], were grown in chambers at current and elevated [CO₂] (400 vs. 700 ppm). Together with nodule and leaf metabolomic profile, characterization of nodule N-fixation and exchange between organs were tested through ¹⁵N₂-labeling analysis. Soybeans inoculated with SFJ14-36 strain (isolated at elevated [CO₂]) showed a strong metabolic imbalance, at nodule and leaf levels when grown at ambient [CO₂], probably due to an insufficient supply of N by nodules, as shown by ¹⁵N₂-labeling. In nodules, due to shortage of photoassimilate, C may be diverted to aspartic acid instead of malate in order to improve the efficiency of the C source sustaining N₂-fixation. In leaves, photorespiration and respiration were boosted at ambient [CO₂] in plants inoculated with this strain. Additionally, free phytol, antioxidants, and fatty acid content could be indicate induced senescence due to oxidative stress and lack of nitrogen. Therefore, plants inoculated with Bradyrhizobium strain isolated at elevated [CO₂] may have lost their capacity to form effective symbiosis at ambient [CO₂] and that was translated at whole plant level through metabolic impairment.

SUBMITTER: Soba D

PROVIDER: S-EPMC8173217 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Publications

Soybean Inoculated With One Bradyrhizobium Strain Isolated at Elevated [CO2] Show an Impaired C and N Metabolism When Grown at Ambient [CO2].

Soba David D Aranjuelo Iker I Gakière Bertrand B Gilard Françoise F Pérez-López Usue U Mena-Petite Amaia A Muñoz-Rueda Alberto A Lacuesta Maite M Sanz-Saez Alvaro A

Frontiers in plant science 20210520

Soybean (Glycine max L.) future response to elevated [CO2] has been shown to differ when inoculated with B. japonicum strains isolated at ambient or elevated [CO2]. Plants, inoculated with three Bradyrhizobium strains isolated at different [CO2], were grown in chambers at current and elevated [CO2] (400 vs. 700 ppm). Together with nodule and leaf metabolomic profile, characterization of nodule N-fixation and exchange between organs ...[more]

PMID: 34093614

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Project description:BackgroundThe present study aims to study the effects of biofertilizers potential of Arbuscular Mycorrhizal Fungi (AMF) and Bradyrhizobium japonicum (B. japonicum) strains on yield and growth of drought stressed soybean (Giza 111) plants at early pod stage (50 days from sowing, R3) and seed development stage (90 days from sowing, R5).ResultsHighest plant biomass, leaf chlorophyll content, nodulation, and grain yield were observed in the unstressed plants as compared with water stressed-plants at R3 and R5 stages. At soil rhizosphere level, AMF and B. japonicum treatments improved bacterial counts and the activities of the enzymes (dehydrogenase and phosphatase) under well-watered and drought stress conditions. Irrespective of the drought effects, AMF and B. japonicum treatments improved the growth and yield of soybean under both drought (restrained irrigation) and adequately-watered conditions as compared with untreated plants. The current study revealed that AMF and B. japonicum improved catalase (CAT) and peroxidase (POD) in the seeds, and a reverse trend was observed in case of malonaldehyde (MDA) and proline under drought stress. The relative expression of the CAT and POD genes was up-regulated by the application of biofertilizers treatments under drought stress condition. Interestingly a reverse trend was observed in the case of the relative expression of the genes involved in the proline metabolism such as P5CS, P5CR, PDH, and P5CDH under the same conditions. The present study suggests that biofertilizers diminished the inhibitory effect of drought stress on cell development and resulted in a shorter time for DNA accumulation and the cycle of cell division. There were notable changes in the activities of enzymes involved in the secondary metabolism and expression levels of GmSPS1, GmSuSy, and GmC-INV in the plants treated with biofertilizers and exposed to the drought stress at both R3 and R5 stages. These changes in the activities of secondary metabolism and their transcriptional levels caused by biofertilizers may contribute to increasing soybean tolerance to drought stress.ConclusionsThe results of this study suggest that application of biofertilizers to soybean plants is a promising approach to alleviate drought stress effects on growth performance of soybean plants. The integrated application of biofertilizers may help to obtain improved resilience of the agro ecosystems to adverse impacts of climate change and help to improve soil fertility and plant growth under drought stress.

Dataset Information

Soybean Inoculated With One Bradyrhizobium Strain Isolated at Elevated [CO₂] Show an Impaired C and N Metabolism When Grown at Ambient [CO₂].

Publications

Soybean Inoculated With One Bradyrhizobium Strain Isolated at Elevated [CO<sub>2</sub>] Show an Impaired C and N Metabolism When Grown at Ambient [CO<sub>2</sub>].

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