Real-Time Measurement of Cellobiose and Glucose Formation during Enzymatic Biomass Hydrolysis.

ABSTRACT: Enzymatic hydrolysis of lignocellulosic biomass for biofuel production relies on complex multi-enzyme ensembles. Continuous and accurate measurement of the released key products is crucial in optimizing the industrial degradation process and also investigating the activity and interaction between the involved enzymes and the insoluble substrate. Amperometric biosensors have been applied to perform continuous cellobiose measurements during the enzymatic hydrolysis of pure cellulose powders. The oxygen-sensitive mediators used in these biosensors restricted their function under physiological or industrial conditions. Also, the combined measurements of the hydrolysis products cellobiose and glucose require a high selectivity of the biorecognition elements. We employed an [Os(2,2'-bipyridine)<

SUBMITTER: Chang H 

PROVIDER: S-EPMC8173519 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature