Immunophenotyping reveals distinct subgroups of lupus patients based on their activated T cell subsets.

ABSTRACT:

Objective

This study performed an integrated analysis of the cellular and transcriptional differences in peripheral immune cells between patients with Systemic Lupus Erythematosus (SLE) and healthy controls (HC).

Methods

Peripheral blood was analyzed using standardized flow cytometry panels. Transcriptional analysis of CD4⁺ T cells was performed by microarrays and Nanostring assays.

Results

SLE CD4⁺ T cells showed an increased expression of oxidative phosphorylation and immunoregulatory genes. SLE patients presented higher frequencies of activated CD38⁺HLA-DR⁺ T cells than HC. Hierarchical clustering identified a group of SLE patients among which African Americans were overrepresented, with highly activated T cells, and high

SUBMITTER: Perry DJ 

PROVIDER: S-EPMC8173542 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature