Increased expression of transient receptor potential channels and neurogenic factors associates with cough severity in a guinea pig model.

ABSTRACT:

Background

Previous studies suggest that transient receptor potential (TRP) channels and neurogenic inflammation may be involved in idiopathic pulmonary fibrosis (IPF)-related high cough sensitivity, although the details of mechanism are largely unknown. Here, we aimed to further explore the potential mechanism involved in IPF-related high cough sensitivity to capsaicin challenge in a guinea pig model of pulmonary fibrosis induced by bleomycin.

Methods

Western blotting and real-time quantitative polymerase chain reaction (RT-qPCR) were employed to measure the expression of TRP channel subfamily A, member 1 (TRPA1) and TRP vanilloid 1 (TRPV1), which may be involved in the cough reflex pathway. Immunohistochemical analysis and RT-qPCR were used to detect the expression of neur

SUBMITTER: Guan M 

PROVIDER: S-EPMC8173754 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature